diff --git a/release/datafiles/userdef/userdef_default.c b/release/datafiles/userdef/userdef_default.c
index 899139e203b..0463399f6ba 100644
--- a/release/datafiles/userdef/userdef_default.c
+++ b/release/datafiles/userdef/userdef_default.c
@@ -21,7 +21,7 @@ const UserDef U_default = {
     .versionfile = BLENDER_FILE_VERSION,
     .subversionfile = BLENDER_FILE_SUBVERSION,
     .flag = (USER_AUTOSAVE | USER_TOOLTIPS | USER_RELPATHS | USER_RELEASECONFIRM |
-             USER_SCRIPT_AUTOEXEC_DISABLE),
+             USER_SCRIPT_AUTOEXEC_DISABLE | USER_FILECOMPRESS),
     .dupflag = USER_DUP_MESH | USER_DUP_CURVE | USER_DUP_SURF | USER_DUP_LATTICE | USER_DUP_FONT |
                USER_DUP_MBALL | USER_DUP_LAMP | USER_DUP_ARM | USER_DUP_CAMERA | USER_DUP_SPEAKER |
                USER_DUP_ACT | USER_DUP_LIGHTPROBE | USER_DUP_GPENCIL | USER_DUP_CURVES |
diff --git a/source/blender/blenkernel/BKE_blender_version.h b/source/blender/blenkernel/BKE_blender_version.h
index ccfc6883980..663c76d7aaf 100644
--- a/source/blender/blenkernel/BKE_blender_version.h
+++ b/source/blender/blenkernel/BKE_blender_version.h
@@ -27,7 +27,7 @@
 
 /* Blender file format version. */
 #define BLENDER_FILE_VERSION BLENDER_VERSION
-#define BLENDER_FILE_SUBVERSION 93
+#define BLENDER_FILE_SUBVERSION 94
 
 /* Minimum Blender version that supports reading file written with the current
  * version. Older Blender versions will test this and cancel loading the file, showing a warning to
diff --git a/source/blender/blenloader/intern/versioning_userdef.cc b/source/blender/blenloader/intern/versioning_userdef.cc
index 59b98641050..611a006e9bc 100644
--- a/source/blender/blenloader/intern/versioning_userdef.cc
+++ b/source/blender/blenloader/intern/versioning_userdef.cc
@@ -1679,6 +1679,11 @@ void blo_do_versions_userdef(UserDef *userdef)
         userdef, "VIEW3D_AST_brush_texture_paint", "Brushes/Mesh Texture Paint/Utilities");
   }
 
+  if (!USER_VERSION_ATLEAST(500, 94)) {
+    /* Force-reset file compression to ON, see #135735. */
+    userdef->flag |= USER_FILECOMPRESS;
+  }
+
   /**
    * Always bump subversion in BKE_blender_version.h when adding versioning
    * code here, and wrap it inside a USER_VERSION_ATLEAST check.
diff --git a/source/blender/makesdna/DNA_userdef_defaults.h b/source/blender/makesdna/DNA_userdef_defaults.h
index d38fe584817..b5aeec7cc17 100644
--- a/source/blender/makesdna/DNA_userdef_defaults.h
+++ b/source/blender/makesdna/DNA_userdef_defaults.h
@@ -19,7 +19,7 @@
 #define _DNA_DEFAULT_bUserAssetLibrary \
   { \
     .import_method = ASSET_IMPORT_APPEND_REUSE, \
-    .flag = ASSET_LIBRARY_RELATIVE_PATH, \
+    .flag = ASSET_LIBRARY_RELATIVE_PATH | USER_FILECOMPRESS, \
   }
 
 /** \} */
diff --git a/source/blender/makesdna/intern/dna_defaults.c b/source/blender/makesdna/intern/dna_defaults.c
index e30a3d9c225..4443a6df0fd 100644
--- a/source/blender/makesdna/intern/dna_defaults.c
+++ b/source/blender/makesdna/intern/dna_defaults.c
@@ -56,7 +56,8 @@
  * - When loading old files that don't contain newly added struct members (these will be zeroed)
  *   to set their values use `versioning_{BLENDER_VERSION}.c` source files.
  * - For startup file data, to update these defaults use
- *   #BLO_update_defaults_startup_blend & #blo_do_versions_userdef.
+ *   #BLO_update_defaults_startup_blend, #blo_do_versions_userdef, and #U_default or
+ *   #U_theme_default.
  */
 
 #define DNA_DEPRECATED_ALLOW